Preparation and Characterization of Hydroxyapatite-Coated Selenium Nanoparticles and their Interaction with Osteosarcoma (SaOS-2) Cells

doi:10.1007/s40195-014-0153-0

Abstract

Abstract:

Nanotechnology has taken a firm step to revolutionize the field of orthopedic implants. Current research on bone implants focuses to develop implants with multifaceted functions viz., osteoinduction, chemoprevention, anti-microbial action etc., especially for cancerous bone resection. The objective of the present study was to synthesize a novel composite for bone implants, possessing the above properties. Selenium was selected owing to its chemopreventive and chemotherapeutic properties. Hydroxyapatite was selected owing to its bioactivity and similarity in composition to bone mineral properties. Selenium nanoparticles were prepared by chemical reduction method and coated with hydroxyapatite. Hydroxyapatite-coated selenium nanoparticle (HASnp) was characterized physico-chemically using fourier transform infrared spectroscopy, X-ray diffractometry, scanning electron microscope, and energy-dispersive X-ray spectroscopy. HASnp was analysed in vitro using SaOS-2 cell line. Enhanced cell proliferation and alkaline phosphatase activity were observed in HASnp-treated cells. The results indicate that HASnp is highly suitable for the use in orthopedic applications.

Key words: Osteogenesis, Biocompatibility, Bone tissue engineering, Biomaterials, Biomedical applications

Hemalatha Thiagarajan, Krithiga Gunasekaran, Santhosh Kumar Baskar, P. Sastry Thotapalli. Preparation and Characterization of Hydroxyapatite-Coated Selenium Nanoparticles and their Interaction with Osteosarcoma (SaOS-2) Cells[J]. Acta Metallurgica Sinica (English Letters), 2014, 27(6): 1152-1158.

Figures/Tables 7

Fig. 1 UV-Vis spectrum of colloidal chitosan-capped selenium nanoparticles

Fig. 2 FTIR spectra of chitosan a, chitosan-capped selenium nanoparticles b, hydroxyapatite-coated selenium nanoparticles c

Fig. 3 SEM images of chitosan-capped selenium nanoparticles a and hydroxyapatite-coated selenium nanoparticles b

Fig. 4 EDX spectra of chitosan-capped selenium nanoparticles a and hydroxyapatite-coated selenium nanoparticles b

Fig. 5 TEM image of colloidal selenium nanoparticles

Fig. 6 X-ray diffraction pattern of hydroxyapatite-coated selenium nanoparticles

Fig. 7 a MTT assay (data are presented as mean ± SD of three individual experiments (n = 3). *p ≤ 0.05 as compared to control group, using Duncan’s multiple range analysis); b Alkaline phosphatase activity (data are presented as mean ± SD of three individual experiments (n = 3). *p ≤ 0.05 as compared to control group, using Duncan’s multiple range analysis)

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