In Vitro Biocompatibility of MC3T3-E1 Osteoblast-like Cells on Arg-Gly-Asp Acid Peptides Immobilized Graphite-like Carbon Coating on Carbon/Carbon Composites

doi:10.1007/s40195-017-0542-2

Abstract

Abstract:

Carbon/carbon (C/C) composites were deposited with graphite-like carbon (GLC) coating, and then, Arg-Gly-Asp acid (RGD) peptides were successfully immobilized onto the functionalized GLC coating. GLC coating was utilized to prevent carbon particles releasing and create a uniform surface condition for C/C composites. RGD peptides were utilized to improve biocompatibility of GLC coating. Surface chemical characterizations of functionalized GLC coating were detected by contact angle measurement, X-ray photoelectron spectroscopy and Raman spectra. Optical morphology of GLC coatings was observed by confocal laser scanning microscopy. In vitro biological performance was determined using samples seeded with MC3T3-E1 osteoblast-like cells and cultured for 1 week. Surface characterizations and morphological analysis indicated that C/C composites were covered by a dense and uniform GLC coating. Contact angle of GLC coating was reduced to 27.2° when it was functionalized by H₂O₂ oxidation at 40 °C for 1 h. In vitro cytological test showed that the RGD peptides immobilized GLC coating had a significant improvement in biocompatibility. It was suggested that RGD peptides provided GLC coating with a bioactive surface to improve cell adhesion and proliferation on C/C composites.

Key words: MC3T3-E1 osteoblast-like cells, Carbon/carbon composites, Graphite-like carbon (GLC) coating, Arg-Gly-Asp acid (RGD) peptides, Surface modification

Sheng Cao, He-Jun Li, Ke-Zhi Li, Jin-Hua Lu, Lei-Lei Zhang. In Vitro Biocompatibility of MC3T3-E1 Osteoblast-like Cells on Arg-Gly-Asp Acid Peptides Immobilized Graphite-like Carbon Coating on Carbon/Carbon Composites[J]. Acta Metallurgica Sinica (English Letters), 2017, 30(6): 558-566.

Figures/Tables 8

Fig. 1 GLC coating on C/C composites: a macrophotograph of C/C composites with GLC coating, b Raman spectra of GLC coating and C/C substrate, c XPS spectra of GLC coating and C/C substrate, d high-resolution C1s spectra of GLC coating and C/C substrate

Fig. 2 Morphology changes in GLC coating at different oxidation temperatures: a 30 °C, b 50 °C, c 60 °C, d 80 °C

Fig. 3 Change trend of Raman spectra of GLC coatings at different oxidation temperatures

Fig. 4 Contact angles of GLC coatings at different oxidation temperatures: a untreated, b 30 °C, c 40 °C, d 50 °C

Fig. 5 XPS spectra of GLC coatings at different oxidation temperatures: a full XPS spectra, b high-resolution C1s and O1s spectra of GLC coating after oxidation at 40 °C

Fig. 6 Cellular morphology of MC3T3-E1 osteoblast-like cells on the modified GLC coating

Fig. 7 In vitro cell adhesion and proliferation of ME3T3-E1 osteoblast-like cells on C/C, GLC, GLC-40 and GLC-RGD specimens

Fig. 8 Schematic diagram of GLC coating peeling at different oxidation temperatures

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