Construction of Nanophase Novel Coatings-Based Titanium for the Enhancement of Protein Adsorption

doi:10.1007/s40195-016-0382-5

Abstract

Abstract:

In the recent years, biological nanostructures coatings have been incorporated into orthopedic and dental implants in order to accelerate osseointegration and reducing surgical restrictions. In the present work, chemical etching, anodization and metal doping surface modification methods were integrated in one strategy to fabricate innovative titanium surfaces denominated by titanium nanoporous, anodized titanium nanoporous, silver-anodized titanium nanoporous and gold-anodized titanium nanoporous. The stability properties of nanostructures-coated surfaces were elucidated using electrochemical impedance spectroscopy (EIS) after 7 days of immersion in simulated biological fluids. Morphology and chemical compositions of new surfaces were characterized by scanning electron microscope and energy-dispersive X-ray analysis. The EIS results and data fitting to the electrical equivalent circuit model demonstrated the influence of adsorption of bovine serum albumin on new surfaces as a function of protein concentration. Adsorption process was described by the very well-known model of the Langmuir adsorption isotherm. The thermodynamic parameter ΔG _ADS (-50 to 59 kJ mol^-1) is calculated, which supports the instantaneous adsorption of protein from biological fluids to new surfaces and refers to their good biocompatibility. Ultimately, this study explores new surface strategy to gain new implants as a means of improving clinical outcomes of patients undergoing orthopedic surgery.

Key words: Titanium, Nanoporous, Surface modification, Protein adsorption, Electrochemical impedance spectroscopy (EIS)

Sahar A. Fadlallah, Mohammed A. Amin, Ghaida S. Alosaimi. Construction of Nanophase Novel Coatings-Based Titanium for the Enhancement of Protein Adsorption[J]. Acta Metallurgica Sinica (English Letters), 2016, 29(3): 243-252.

Figures/Tables 8

References 47

[1]	W.Q. Yu, Y.L. Zhang, X.Q. Jiang, F.Q. Zhang, Oral Dis. 16, 624(2010)
[2]	C.C. Mohan, K.P. Chennazhi, D. Menon, Acta Biomater. 9, 9568(2013)
[3]	W.Q. Yu, J. Qiu, F.Q. Zhang, Colloids Surf. B 84, 400 (2011)
[4]	A. Wennerberg, T. Albrektsson, C. Johansson, B. Andersson, Biomaterials 17, 15 (1996)
[5]	H.W. Kim, Y.H. Koh, L.H. Li, S. Lee, H.E. Kim, Biomaterials 25, 2533 (2004)
[6]	S. Nishiguchi, H. Kato, H. Fujita, M. Oka, H.M. Kim, T. Kokubo, T. Nakamura, Biomaterials 22, 2525 (2001)
[7]	H.J. Song, S.H. Park, S.H. Jeong, Y.J. Park, J. Mater. Process. Technol. 209, 864(2009)
[8]	J.I. Lim, B. Yu, K.M. Woo, Y.K. Lee, Appl. Surf. Sci. 255, 2456(2008)
[9]	A. Al-Ahmad, M.W. Al-Ahmad, A. Fackler, M. Follo, E. Hellwig, M. Bächle, C. Hannig, J.S. Han, M. Wolkewitz, R. Kohal, Arch. Oral Biol. 58, 1139(2013)
[10]	J.J. Ryu, S. Letchuman, P. Shrotriya, J. Mech. Behav. Biomed. Mater. 14(5), 55(2012)
[11]	F. Rupp, R.A. Gittens, L. Scheideler, A. Marmur, B.D. Boyan, Z. Schwartz, J.G. Gerstorfer, Acta Biomater. 10, 2894(2014)
[12]	S. Wu, X. Liu, W.K. Yeung, H. Guo, P. Li, T. Hu, C.Y. Chung, P.K. Chu, Surf. Coat. Technol. 233, 13(2013)
[13]	N. Wang, H. Li, W. Lü, J. Li, J. Wang, Z. Zhang, Y. Liu, Biomaterials 32, 6900 (2011)
[14]	Y. Shibata, Y. Tanimoto, J. Prosthodont. Res. 59, 20(2015)
[15]	Y. Tian, H. Cao, Y. Qiao, F. Meng, X. Liu, Acta Biomater. 10, 4505(2014)
[16]	H. Wu, X. Zhang, X. He, M. Li, X. Huang, R. Hang, B. Tang, Appl. Surf. Sci. 317, 614(2014)
[17]	H. Li, Q. Cui, B. Feng, J. Wang, X. Lu, J. Weng, Appl. Surf. Sci. 284, 179(2013)
[18]	G. Mani, D.M. Johnson, D. Marton, M.D. Feldman, D. Patel, A.A. Ayon, C. Mauli Agrawal, Biomaterials 29, 4561(2008)
[19]	S.M.F. Gad El-Rab, S.A. Fadl-allah, A.A. Montser, Appl. Surf. Sci. 261, 1(2012)
[20]	J.A. Lyndon, B.J. Boyd, N. Birbilis, J. Control. Release 179, 63 (2014)
[21]	S.B. Goodman, Z. Yao, M. Keeney, F. Yang, Biomaterials 34, 3174 (2013)
[22]	M.M. Speeckaert, R. Speeckaert, N.V. Geel, J.R. Delanghe, Adv. Clin. Chem. 63, 1(2014)
[23]	K. Mark, J. Park, Mater. Sci. 58, 327(2013)
[24]	J.Y. Lim, M.C. Shaughnessy, Z. Zhou, H. Noh, E.A. Vogler, H.J. Donahue, Biomaterials 29, 1778 (2008)
[25]	S. Sista, C. Wen, P.D. Hodgson, G. Pande, Mater. Sci. Eng. C 33, 1573 (2013)
[26]	P. Silva-Bermudez, S.E. Rodil, Surf. Coat. Technol. 233, 147(2013)
[27]	W.A. Badawy, A.M. Fathi, R.M. El-Sherief, S.A. Fadl-Allah, J. Alloys Compd. 475, 911(2009)
[28]	S.A. Fadl-allah, Q. Mohsen, Appl. Surf. Sci. 256, 5849(2010)
[29]	C. Matschegewski, S. Staehlke, R. Loeffler, R. Lange, F. Chai, D.P. Kern, U. Beck, B.J. Nebe, Biomaterials 31, 5729 (2012)
[30]	N.S. El-Shenawy, Q. Mohsen, S.A. Fadl-allah, J. Mater. Sci. Mater. Med. 23, 1763(2012)
[31]	N.I.Z. Abidin, A.D. Atrens, D. Martin, A. Atrens, Corros. Sci. 53, 3542(2011)
[32]	Q. Zhao, T. Cao, Mater. Sci. Eng. B 172, 163 (2010)
[33]	F.M.B. Hassan, H. Nanjo, S. Venkatachalam, M. Kanakubo, T. Ebina, Electrochim. Acta 55, 3130 (2010)
[34]	C.F. Almeida Alves, F. Oliveira, I. Carvalho, A.P. Piedade, S. Carvalho, Mater. Sci. Eng. C 34, 22 (2014)
[35]	Y. Zhang, R. Yuan, Y. Chai, Y. Xiang, X. Qian, H. Zhang, J. Colloid Interface Sci. 341, 108(2010)
[36]	P.D.V. ConCellos, S. Bose, H. Beyenal, A. Bandyopadhyay, L.G. Zirkle, Mater. Sci. Eng. C 32, 1112 (2012)
[37]	S. Eraković, A. Janković, C. Ristoscu, L. Duta, N. Serban, A. Visan, I.N. Mihailescu, G.E. Stan, M. Socol, O. Iordache, I. Dumitrescu, C.R. Luculescu, D.J. Janaćković, V.M. Stanković, Appl. Surf. Sci. 293, 37(2014)
[38]	U. Rammelt, N. Hebestreit, A. Fikus, W. Plieth, Electrochim. Acta 46, 2363 (2001)
[39]	L. Zhao, H. Wang, K. Huo, L. Cui, W. Zhang, H. Ni, Y. Zhang, Z. Wu, P.K. Chu, Biomaterials 32, 5706 (2011)
[40]	R.K. Mohanty, S. Thennarasu, A.B. Mandal, Colloids Surf. B 114, 138 (2014)
[41]	H. Cai, P. Yao, Colloids Surf. B 123, 900 (2014)
[42]	W. Wang, F. Mohammadi, A. Alfantazi, Corros. Sci. 57, 11(2012)
[43]	D. Kowalski, D. Kim, P. Schmuki, Nano Today 8, 235 (2013)
[44]	S. Karimi, T. Nickchi, A. Alfantazi, Corros. Sci. 53, 3262(2011)
[45]	D.C. Hansen, G.W. Luther, J.H. Waite, J. Colloid Interface Sci. 168, 206(1994)
[46]	Z. Rong, Y. Zhao, B. Liu, Y. Tian, B. Yang, J. Electroanal. Chem. 707, 102(2013)
[47]	J. Zhang, A.M. Bond, J. Electroanal. Chem. 574, 299(2005)

Sample	Ti	O	C	Cl	Ag	Au	N	Na
Ti	100	-	-	-	-	-	-	-
TNP	97.12	-	-	-	-	-	2.88	-
ATNP	84.28	15.72	-	-	-	-	-	-
Ag-ATNP	77.10	17.40	-	-	1.60	-	-	-
Au-ATNP	80.85	17.72	-	-	-	1.72	-	-

Sample	Ti	O	C	Cl	Ag	Au	N	Na
Ti	100	-	-	-	-	-	-	-
TNP	97.12	-	-	-	-	-	2.88	-
ATNP	84.28	15.72	-	-	-	-	-	-
Ag-ATNP	77.10	17.40	-	-	1.60	-	-	-
Au-ATNP	80.85	17.72	-	-	-	1.72	-	-

Sample	Ti	O	C	Cl	Ag	Au	N	Na
Ti	75.47	-	10.54	2.52	-	-	7.40	4.44
TNP	88.95	-	-	-	-	-	11.05	-
ATNP	90.72	-	-	-	-	-	9.28	-
Ag-ATNP	90.59	-	-	-	-	-	9.41	-
Au-ATNP	89.88	-	-	-	-	-	10.12	-

Sample	Ti	O	C	Cl	Ag	Au	N	Na
Ti	75.47	-	10.54	2.52	-	-	7.40	4.44
TNP	88.95	-	-	-	-	-	11.05	-
ATNP	90.72	-	-	-	-	-	9.28	-
Ag-ATNP	90.59	-	-	-	-	-	9.41	-
Au-ATNP	89.88	-	-	-	-	-	10.12	-

Implant surface	BSA concentration (g/L)	R _s(Ω)	Q _p	R _p(kΩ cm²)	n _p	C _p(µF/cm²)	Q _b	R _b(kΩ cm²)	n _b	C _b(µF/cm²)	R _ct(kΩ cm²)	θ(°)	χ ²
Ti	0	10.8	18.6	631	0.89	25.24	577.4	15	0.78	1061.6	646	77.8	0.1
	0.2	14.9	15.81	980	0.99	16.25	797.6	16	0.92	995.2	996	81.8	0.4
	2.9	18	15.14	989	0.98	16.1	929.7	11	0.84	1447.6	1000	78.9	0.2
	6	19.1	13.31	986	0.93	16.15	255.1	17	0.53	936.7	1003	81.7	0.04
TNP	0	10	19.86	780	0.99	20.42	572.2	16	0.8	995.2	796	78	0.08
	0.2	10.3	11.9	984	0.89	16.18	653.6	17	0.87	936.7	1001	80.2	0.9
	2.9	10.5	15.2	990	0.98	16.08	444.4	25	0.87	636.9	1015	81.6	0.9
	6	9.7	14.04	988	0.95	16.12	427.8	22	0.81	723.8	1010	82.6	0.9
ATNP	0	24.4	3.87	4000	0.99	3.98	61.05	150	0.8	106.2	4150	85.8	0.6
	0.2	68	2.89	4300	0.91	3.7	70.75	166	0.89	95.93	4466	84.6	0.9
	2.9	36	3	3600	0.86	4.42	28.95	172	0.58	92.6	3772	81.3	0.9
	6	41.5	2.21	7000	0.99	2.27	45.58	190	0.78	83.81	7190	80.2	0.02
Ag-ATNP	0	35.4	2.52	5000	0.93	3.06	12.46	200	0.63	79.62	5200	83	0.05
	0.2	33.7	3.11	4460	0.95	3.57	33.97	255	0.78	62.45	4715	83.1	0.9
	2.9	38.5	3.26	4500	0.97	3.54	39.33	260	0.84	61.24	4760	81.1	0.4
	6	29	2.2	4400	0.82	3.62	33.91	270	0.8	58.98	4670	83.9	0.9
Au-ATNP	0	35	1.88	8000	0.98	1.99	39.42	149	0.64	106.7	8149	83.1	0.3
	0.2	32.9	2.04	6600	0.94	2.41	37.25	220	0.76	72.38	6820	84.6	0.5
	2.9	31.7	1.85	7500	0.95	2.12	28.19	275	0.74	57.9	7775	81.3	0.8
	6	31.3	1.73	7800	0.94	2.04	41.94	280	0.89	56.87	8080	80.2	0.1