Doping Gd3+ Ion in PDA-PHBV Coating on Ti6Al4V Alloy for Enhancing Corrosion Resistance and Proliferation of Human Gingival Fibroblasts and Human Umbilical Vein Endothelial Cells

doi:10.1007/s40195-021-01292-4

Abstract

Abstract:

In this study, we fabricated poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) coatings doped with Gd³⁺ (1, 5, and 10 × 10^-4 mol/L) on Ti6Al4V alloy for the first time to promote soft tissue sealing around dental implants. The corrosion resistance of Gd³⁺-modified PHBV-coated Ti6Al4V was studied by electrochemical and immersion tests, respectively, whereas CCK-8 and RT-PCR evaluated the biocompatibility to human gingival fibroblasts (HGFs) and human umbilical vein endothelial cells (HUVECs). It was found that the Gd³⁺-modified PHBV coating could enhance the corrosion resistance of Ti6Al4V. In vitro cell tests showed that PHBV coatings with and without Gd³⁺ addition could promote adhesion and proliferation of HGFs and HUVECs, showing a Gd³⁺ content-dependent manner. Moreover, it was found that the PDA-PHBV@1Gd showed the best proliferation to HGFs by up-regulating gene expressions of VINCULIN, ITGB1, and ITGA3, whereas the best response to HUVECs with the highest gene expression of eNOS and HIF-1α genes was found in the PDA-PHBV@5Gd-coated group.

Key words: Soft tissue sealing, Poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV), Gd³⁺, Corrosion resistance, Human gingival fibroblasts

Sijie Qin, Xiongcheng Xu, Yanjin Lu, Liu Li, Tingting Huang, Jinxin Lin. Doping Gd3+ Ion in PDA-PHBV Coating on Ti6Al4V Alloy for Enhancing Corrosion Resistance and Proliferation of Human Gingival Fibroblasts and Human Umbilical Vein Endothelial Cells[J]. Acta Metallurgica Sinica (English Letters), 2022, 35(5): 812-824.

Figures/Tables 13

References 61

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Chemical compound	Abbreviation	Quality
Sodium chloride	NaCl	8.035 g
Sodium bicarbonate	NaHCO₃	0.3550 g
Potassium chloride	KCl	0.2250 g
Potassium hydrogen phosphate trihydrate	KHPO₃·3H₂O	0.2310 g
Magnesium chloride hexahydrate	MgCl·6H₂O	0.3110 g
hydrochloric acid (mol/L)	HCl (1 mol/L)	39.00 mL
Calcium chloride	CaCl₂	0.2920 g
Sodium sulfate	Na₂SO₄	0.07200 g
Tris (hydroxymethyl) aminomethane	(CHOH)₃CNH₂	6.118 g
hydrochloric acid (1 mol/L)	HCl (1 mol/L)	0-5 mL

Chemical compound	Abbreviation	Quality
Sodium chloride	NaCl	8.035 g
Sodium bicarbonate	NaHCO₃	0.3550 g
Potassium chloride	KCl	0.2250 g
Potassium hydrogen phosphate trihydrate	KHPO₃·3H₂O	0.2310 g
Magnesium chloride hexahydrate	MgCl·6H₂O	0.3110 g
hydrochloric acid (mol/L)	HCl (1 mol/L)	39.00 mL
Calcium chloride	CaCl₂	0.2920 g
Sodium sulfate	Na₂SO₄	0.07200 g
Tris (hydroxymethyl) aminomethane	(CHOH)₃CNH₂	6.118 g
hydrochloric acid (1 mol/L)	HCl (1 mol/L)	0-5 mL

Samples	E_corr (mV)	I_corr (nA cm^-2)	Rate_corr (mpy)
Ti6Al4V	731.0 ± 5.312	377.8 ± 3.213	(172.6 ± 2.601) × 10^-3
PDA-PHBV	296.1 ± 4.056	35.50 ± 4.562	(16.65 ± 2.031) × 10^-3
PDA-PHBV@1Gd	196.2 ± 4.321	32.60 ± 4.026	(14.88 ± 2.956) × 10^-3
PDA-PHBV@5Gd	545.2 ± 4.956	30.51 ± 4.701	(14.00 ± 3.052) × 10^-3
PDA-PHBV@10Gd	766.3 ± 5.012	23.42 ± 3.978	(10.51 ± 2.986) × 10^-3

Samples	E_corr (mV)	I_corr (nA cm^-2)	Rate_corr (mpy)
Ti6Al4V	731.0 ± 5.312	377.8 ± 3.213	(172.6 ± 2.601) × 10^-3
PDA-PHBV	296.1 ± 4.056	35.50 ± 4.562	(16.65 ± 2.031) × 10^-3
PDA-PHBV@1Gd	196.2 ± 4.321	32.60 ± 4.026	(14.88 ± 2.956) × 10^-3
PDA-PHBV@5Gd	545.2 ± 4.956	30.51 ± 4.701	(14.00 ± 3.052) × 10^-3
PDA-PHBV@10Gd	766.3 ± 5.012	23.42 ± 3.978	(10.51 ± 2.986) × 10^-3

Samples	R₁ (Ω cm²)	R₂ (MΩ cm²)	Z_CPE (μF cm^-2)	n	χ²
Ti6Al4V	33.90 ± 2.350	0.207 ± 0.014	41.52 ± 0.505	0.902 ± 0.003	1.201 × 10^-4
PDA-PHBV	25.28 ± 1.905	0.451 ± 0.020	82.67 ± 0.804	0.912 ± 0.006	1.211 × 10^-4
PDA-PHBV@1Gd	37.10 ± 2.032	0.631 ± 0.014	78.02 ± 0.780	0.920 ± 0.006	1.024 × 10^-4
PDA-PHBV@5Gd	47.56 ± 2.102	0.788 ± 0.016	85.32 ± 1.353	0.924 ± 0.006	1.204 × 10^-4
PDA-PHBV@10Gd	36.70 ± 1.956	0.995 ± 0.020	79.74 ± 1.078	0.923 ± 0.007	1.101 × 10^-4