Green Synthesis of Silver Nanoparticles and Their Reduced Graphene Oxide Nanocomposites as Antibacterial Agents: A Bio-inspired Approach

doi:10.1007/s40195-016-0485-z

Abstract

Abstract:

In this paper, silver nanoparticles (AgNPs) and AgNPs/reduced graphene oxide (RGO) nanocomposites were prepared using lemon juice under microwave irradiation (MWI) and UV light irradiation. AgNPs with face-centered cubic structure RGO peaks were observed by X-ray diffraction. The UV-Vis spectrum showed modifications in the absorption peaks of the AgNPs with the concentration of the precursor solution and irradiation time, and the optimized condition was obtained for 20 min MWI and 60 s of UV light. Raman analysis confirmed the presence of RGO as D and G bands in the spectrum. Transmission electron microscopy analyses confirmed that the AgNPs of size ranging from 3 to 8 nm were anchored onto the RGO sheets. The antibacterial properties of the AgNPs/RGO nanocomposites were investigated using gram-negative bacteria. The results revealed that AgNPs/RGO nanocomposites consisting of approximately 5 wt% AgNPs can achieve antibacterial performance similar to that of neat AgNPS. This method can be useful for the applications of AgNPs-based nanocomposites, where minute amount of silver will be utilized.

Key words: Silver nanoparticles (AgNPs), Nanocomposites, Reduced graphene oxide, Antibacterial

Edreese Alsharaeh,Sarah Alazzam,Faheem Ahmed,Nishat Arshi,Mohammed Al-Hindawi,Garwin Kim Sing. Green Synthesis of Silver Nanoparticles and Their Reduced Graphene Oxide Nanocomposites as Antibacterial Agents: A Bio-inspired Approach[J]. Acta Metallurgica Sinica (English Letters), 2017, 30(1): 45-52.

Figures/Tables 9

Fig. 1 UV-Vis spectra of AgNPs using lemon with various concentrations and processed using MWI times of: a 40 s, b 60 s, and c 80 s. d Change in color of AgNPs formed by MWI for 40, 60 and 80 s

Fig. 2 UV-Vis spectra of AgNPs prepared using lemon with different concentrations and processed in UV light for: a 20 min, b 30 min

Fig. 3 XRD patterns of a AgNPs with different concentrations (1-5 mM) using MWI for 60 s, b AgNPs/RGO nanocomposites prepared from 2 mM under MWI for 60 s

Fig. 4 TEM images of a AgNPs prepared from 2 mM under MWI for 60 s, b AgNPs prepared from 2 mM under UV light for 20 min

Fig. 5 UV-Vis spectra for AgNPs/RGO nanocomposites prepared using: a MWI for 60 s, b UV light for 20 min

Fig. 6 TEM images of a AgNPs/RGO nanocomposites prepared from 2 mM under MWI for 60 s, b AgNPs/RGO prepared from 2 mM under UV light for 20 min and c EDX spectrum of the AgNPs/RGO sample using a 2 mM concentration under MWI for 60 s

Fig. 7 Raman spectrum of AgNPs/RGO nanocomposites using a 2 mM concentration under MWI for 60 s

Fig. 8 Inhibition zones for a RGO alone, AgNPs for various concentrations (1-5 mM) using b-d MWI for 60 s and e-g UV light irradiation for 20 min

Fig. 9 Inhibition zones for AgNPs/RGO nanocomposites for various concentrations (1-5 mM) using a-c MWI for 60 s and d-e UV light irradiation for 20 min

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